Basic Info.
Supply Type
OBM(Original Brand Manufacturing)
Usage Mode
For external use
Type
Biological Diagnostic Reagents
Application
for Diagnosis
Transport Package
by Foam Box with Ice to Maintain Cool Storage
Specification
96wells/kit
Product Description
Ochratoxin A(OTA)ELISA Test Kit
Catalog No. LSY-10034
1. General
Ochratoxin A are secondary metabolites of Aspergillus and Penicillium strains, exist widely in grain and grain products, spices and coffee beans and other products. Ochratoxin A has a strong liver and kidney toxicity, and has teratogenic, mutagenic and carcinogenic effects, which is serious harmful to human health.
Thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) has been a common method of Ochratoxin A detection, but sample preparation and samples analysis of this method is time-consuming and exhausting. The use of Ochratoxin A ELISA kit to analyze Ochratoxin A residue of samples is fast and accurate.
This kit is a new generation of fungal toxins detection products in application of ELISA technology, to minimize operating errors and work intensity.
2. Principle
The test is based on a direct competitive enzyme immunoassay method. The microtiter wells are coated with Ochratoxin antigen. Free Ochratoxin in sample solutions and the immobilized antigen compete for Ochratoxin enzyme conjugate.
While Ochratoxin antibody and HRP secondary antibody (HRP material) combined, via the TMB substrate show color, sample absorbance values of Ochratoxin has a negative correlation with its content, compared with the standard curve and then multiplied by the corresponding the dilution factor, can draw Ochratoxin content in samples.
3. Application
This test kit can be used to detect Ochratoxin in Corn, wheat and other grain samples qualitatively and quantitatively.
4. Technical specifications
Sensitivity: 0.1 ppb
Detection limit:
Corn, whole wheat ...........................…1ppb
Recovery rate:
Corn .....................................80±10%
Whole wheat...........................….…..90±10%
Cross-reaction rate
Ochratoxin A .................................100%
Ochratoxin B .................................9.3%
Ochratoxin C ..................................46%
Coefficient of variation: <10%
5. Components
1) OTA Micro-well strips: 12 strips with 8 removable wells each
2) 5 x Concentrated standard (3x) ...............….1 ml each
(Remark: Concentrated standard (3x) is 0 ppb (zero standard), 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb; Working
Standard is 0ppb, 0.1ppb, 0.3ppb, 0.9ppb, 2.7ppb)
3) OTA Enzyme-labled conjugate .............................................. 12 ml
4) OTA Antibody ........................................................................... 6ml
5) Substrate A .............................................................................. 6ml
6) Substrate B................................................................................ 6ml
7) Stop solution............................................................................... 6ml
8) Concentrated wash buffer(10×)...........................................40ml
Address:
D Building, National Biological Industrial Park of Marinelife, No. 2 Binhai Road, Dapeng, Shenzhen, Guangdong, China
Business Type:
Manufacturer/Factory, Trading Company, Other
Business Range:
Agriculture & Food, Chemicals, Health & Medicine, Industrial Equipment & Components, Security & Protection, Service
Management System Certification:
ISO 9001, ISO 14000
Company Introduction:
Shenzhen Lvshiyuan Biotechnology Co., Ltd., is a high-tech enterprise, established by a senior scholars who had studied in America for many years in December, 2003. The company has set up cooperation relationship with China agricultural University, Central China Agricultural University, Harbin Veterinary Laboratory, etc; And primarily focuses on developing the most advanced animal disease diagnostic kit, food safety ELISA test kit, environmental monitoring reagent, new enzyme reagent, microorganism reagent, protein product, nucleotide product, green aquatic drugs product, small molecular antigen, antibody, biochemistry product, etc. We run enterprise with the first-class internationalization idea of enterprise management, abundant research-development strength, standardized production criterion, perfect sales network, complete system of technical service and broad domestic-foreign cooperation network to serve the whole world, to promote biological reagent in worldwide industrialization.
Shenzhen Lvshiyuan Biotechnology Co., Ltd., is located longgang Overseas Venture Park, Shenzhen City, China. With doctors and masters as the nucleus regiment and above 95% graduates consist of the specialists of production and research-development, The company is equipped with compete research-development and production platform of antigen, antibody, test kit, test paper, biosensor, cellular engineering, enzyme engineering, genetic engineering. At present, we manufacture food safety test kits (for example: Florfenicol ELISA Test Kit, ELISA Test Kit, Nitrofuran Test kit), Animal disease Diagnostic kits(for example: Porcine Parovirus ELISA Test Kit, Porcine circovirus ELISA Test kit, Swine foot and Mouth Disease Antibody Test Kit, etc), molecular biological special tool enzyme, green aquatic drug product, wich are certificated by ISO9001: 2000, is widely employed in the state agency, enterprise, scientific research institution, etc.
The 21st century is the biological high-tech times, we take "building up green world, spring from you and our cooperation" as the goal, insist that "Biotechnology serving humanity" as the management ideal, provide the leading biological technology product and the perfect technical service for the customers.